Trends in methodology for vancomycin and flucytosine assays as illustrated by numbers of laboratories using a particular method
| Number of users | ||||||
|---|---|---|---|---|---|---|
| Year | Bioassay (±) | FPIA | EMIT | HPLC | Others | Total |
| For bioassay, ± indicates numbers for Gram positive and Gram negative plate, respectively, and * indicates a yeast bioassay technique. | ||||||
| Other methodologies include Opus thin film immunoassay, ACS immunoassay. | ||||||
| EMIT, a commercial enzyme immunoassay for gentamycin; FPIA, fluorescence polarisation immunoassay; HPLC, high performance liquid chromatography; NA, not applicable to this analyte. | ||||||
| Vancomycin | ||||||
| 1984 | 27/0 | 17 | 0 | 0 | 0 | 44 |
| 1985 | 24/0 | 29 | 0 | 0 | 0 | 53 |
| 1991 | 4/0 | 118 | 15 | 1 | 0 | 138 |
| 1993 | 2/0 | 137 | 13 | 1 | 0 | 153 |
| 1997 | 3/0 | 173 | 8 | 0 | 3 | 187 |
| 1999 | 3/0 | 202 | 5 | 1 | 7 | 218 |
| Flucytosine | ||||||
| 1991 | 5* | NA | NA | 8 | 0 | 13 |
| 1993 | 5* | NA | NA | 7 | 0 | 12 |
| 1997 | 7* | NA | NA | 5 | 0 | 12 |
| 1999 | 8* | NA | NA | 8 | 0 | 16 |