Primers used for the polymerase chain reaction
| Target DNA | Primer | Amplicon (bp) | Ref. | |
|---|---|---|---|---|
| CMV, cytomegalovirus; HHV, human herpesvirus; HPV, human papillomavirus; ORF, open reading frame. | ||||
| CMV morphological transforming region II | Outer | CMTR1: 5′-CTG TCG GTG ATG GTG TCT TC-3′; CMTR2: 5′-CCC GAC ACG CGG AAA AGA AA-3′ | 230 | 22 |
| Inner | CMTR3: 5′-TCT CTG GTC CTG ATC GTC TT-3′; CMTR4: 5′-GTG ACC TAC CAA CGT AGG TT-3′ | 168 | ||
| HHV-6 major capsid protein encoding region | Outer | H6-6: 5′-AAG CTT GCA CAA TGC CAA AAA ACA G-3′; H6-7: 5′-CTC GAG TAT GCC GAG ACC CCT AAT C-3′ | 223 | 23 |
| Inner | NH-6: 5′-TCC ATT ATT TTG GCC GCA TTC GT-3′; NH-7: 5′-TGT TAG GAT ATA CCG ATG TGC GT-3′ | 130 | 24 | |
| HHV-7 U10 region | Outer | P1: 5′-TAT CCC AGC TGT TTT CAT ATA GTA AC-3′; P2: 5′-GCC TTG CGG TAG CAC TAG ATT TTT TG-3′ | 186 | 25 |
| Inner | P3: 5′-CAG AAA TGA TAG ACA GAT GTT GG-3′; P4: 5′-TAG ATT TTT TGA AAA AGA TTT AAT AAC-3′ | 124 | ||
| HPV L1 ORF | MY09: 5′-CGT CCM ARR GGA WAC TGA TC-3′ | 450 | 26 | |
| MY11: 5′-GCM CAG GGW CAT AAY AAT GG-3′ (M = A or C; R = A or G; W = A or T; Y = C or T) | ||||