Positive rates of human papillomavirus (HPV) PCRs according to DNA extraction methods
| No. of HPV DNA positive by | |||
|---|---|---|---|
| Extraction method | No. of samples | MY09/11 PCR (450 bp) | GP5+/6+ PCR (150 bp) |
| Phenol/chloroform | |||
| 509 bp β globin PCR positive | 10 | 2 | 5 |
| 509 bp β globin PCR negative | 10 | 3 | 7 |
| Microwave | |||
| 509 bp β globin PCR positive | 12 | 8 | 10 |
| 509 bp β globin PCR negative | 8 | 2 | 2 |
| Qiagen spin column | |||
| 509 bp β globin PCR positive | 10 | 9 | 9 |
| 509 bp β globin PCR negative | 10 | 4 | 6 |