Loss of heterozygosity at eight polymorphic markers in 81 patients with invasive breast tumours
| Microsatellite with previous LOHfrequency | Chromosomal region | Sequences of the primers |
|---|---|---|
| egfr, epidermal growth factor receptor gene. | ||
| D6S261 | 6q21–23 | Al6261 A: 5`-FAM-TGG GCA ATA TAG TGA AAC CCT GTC |
| 18% (10%) | Al6261 B: 5`-TTA TAG GAC CAT GCC ATT AGG CC | |
| D6S300 | 6q 16–21 | Al6300 A: 5`-FAM-TGA AGA TAC ATT GAC ATT CTT CCG C |
| 26% (10%) | Al6300 B: 5`-AGC TCC ACC GTT AAA GAG TAA ATC TG | |
| D8S272 | 8q24.13–qter | Al8272 A: 5`-HEX-AAT GCA CAC ACT CTT ACA CAC AAC G |
| 25% (11%) | Al8272 B: 5`-TGT GGT TAA TCT TGA GAG GTT CTT TG | |
| D11S907 | 11p13 | Al11907 A: 5`-FAM-TGT CCA TAC CCA AAT CGT TCT CTT |
| 11% (12%) | Al11907 B: 5`-GGC AGT GGG ATA TCA GTC CAG TT | |
| D11S925 | 11q23.3–24 | Al11925 A: 5`-HEX-GCT CCT CCA GTA ATT CTG TCC AGA C |
| 26% (12%) | Al11S925 B: 5`-GCT CTT TGA AAC TTG AAA GTG CTA C | |
| D11S927 | 11q22–23 | Al11S927A: 5`-HEX-GTG CCA GAG CGA GAC TCC GT |
| 32% (12%) | Al11S927B: 5`-TGC TGT CTT GAA ACC AAA CCA AAA GCC | |
| Polymorphic marker intron 1 egfr | 7p12–13 | BB1: GTT TGA AGA ATT TGA GCC AAC C |
| 39% (31%) | BB2: TTC TTC TGC ACA CTT GGC AC | |
| p53BECA1 | 17p13 | p53BECA1: FAM-AAG AAA TTC CCA CTG CCA CTC C |
| 49% (32%) | p53BECA2: ATC CCC TGA GGG ATA CTA TTC AGC | |