MPO-ANCA and PR3-ANCA ELISA, ANCA Combi-kit™ ELISA, and ANCA IIF results
| IIF staining pattern (titre) on ethanol fixed neutrophils using polyclonal FITC conjugated antihuman IgG | |||||
|---|---|---|---|---|---|
| Sera | MPO-ANCA and PR3-ANCA IgG ELISA (U/ml) | ANCA Combi-kit IgG ELISA (OD ratio) | Inova Diagnostics | MBL | In house cytospin |
| ORGenTec MPO-ANCA and PR3-ANCA IgG ELISA: positive, >5 U/ml; negative, ≤5 U/ml. | |||||
| ORGenTec ANCA Combi-kit® IgG ELISA OD ratio: positive, ≥1; negative, <1 (only positive results shown). | |||||
| IIF staining pattern: C, classic granular cytoplasmic IIF staining with central/interlobular accentuation. P, perinuclear. | |||||
| Sera 1–6 were from patients with biopsy confirmed microscopic polyangiitis. | |||||
| Serum 7 was from a patient with biopsy confirmed Wegener's granulomatosis. | |||||
| ANCA, antineutrophil cytoplasmic antibody; ELISA, enzyme linked immunosorbent assay; FITC, fluorescein isothiocyanate; IIF, indirect immunofluorescence; MPO, myeloperoxidase; OD, optical density; PR3, proteinase 3. | |||||
| 1 | MPO positive (58) | MPO (6.4) | P (1/160) | P (1/160) | P (1/160) |
| PR3 negative | Atypical cytoplasmic (1/160) | ||||
| 2 | MPO positive (61) | MPO (2.9) | P (1/160) | P (1/160) | P (1/160) |
| PR3 negative | Atypical cytoplasmic (1/10) | ||||
| 3 | MPO positive (8) | MPO (1.6) | P (1/40) | P (1/160) | P (1/160) |
| PR3 negative | Atypical cytoplasmic (1/40) | ||||
| 4 | MPO positive (6) | MPO (1.3) | P (1/10) | P (1/40) | P (1/40) |
| PR3 negative | Atypical cytoplasmic (1/40) | ||||
| 5 | MPO positive (>100) | MPO (9.1) | P (1/160) | P (1/640) | P (1/160) |
| PR3 negative | Lysozyme (1.7) | Atypical cytoplasmic (1/160) | |||
| 6 | MPO positive (36) | MPO (5.2) | P (1/160) | P (1/160) | P (1/160) |
| PR3 negative | Lactoferrin (1.1) | Atypical cytoplasmic (1/10) | |||
| 7 | MPO negative | PR3 (6.44) | C (1/40) | C (1/40) | C (1/40) |
| PR3 positive (53) | |||||