Antibody | Mouse isotype | Immunogen | Specificity | Material and staining dilution | Refs |
---|---|---|---|---|---|
E29 and 139H2 detect MUC1 irrespective of the degree of glycosylation. | |||||
DF3 also detects MUC1 irrespective of the degree of glycosylation, with the exception of strongly hyperglycosylated MUC1, as present in small intestine. | |||||
VU-4H5 detects MUC1 when the threonine residue is non-glycosylated. | |||||
SM3 detects MUC1 when it is hypoglycosylated; that is, contains short sugar chains. | |||||
EMA, epithelial membrane antigen. | |||||
E29 (anti-EMA) | IgG2a | Human milk fat globule membrane | APDTRP | Purified protein, 1/1000 | 26, 37 |
139H2 | IgG1 | Human milk fat globule membrane | APDTRPAP | Ascites material, 1/1000 | 46 |
DF3 | IgG1 | Membrane from breast metastasis | APDTRPAP | Purified protein, 1/1000 | 26, 47 |
VU-4H5 | IgG1 | 60 mer MUC1 peptide–BSA | APDTRPAP | Ascites material, 1/1000 | 48 |
SM3 | IgG1 | Deglycosylated milk mucin | APDTRP | Culture supernatant, undiluted | 26, 38 |