Table 2

Primers and polymerase chain reaction (PCR) conditions

Primers	Sequences	Genes	Size of anplified DNA	MgCl₂ concentration	PCR conditions
HCMV, human cytomegalovirus; HSV, herpes simplex virus; VZV, varicella zoster virus.
HSV-1TK	TkR`-5′tcagttagcctcccccatc	Thymidine kinase	1130 bp	1 mM	95°C 1 min, 55°C 1 min, 72°C 1 min (35 cycles), and 72°C 15 min
	TkF`-5′atggcttcgtacccctgcc
VZV-TK	TkR`-5′aggaagtgttgtcctgaacggc	Thymidine kinase	1024 bp	2 mM	95°C 1 min, 57°C 1 min, 72°C 1 min (35 cycles), and 72°C 15 min
	TkF`-5′atgtcaacggataaaaccgatgt
HCMV	PolF-5′gctatgtttcagatgtcgccgcc	DNA polymerase	170 bp	1.5 mM	95°C 1 min, 62°C 1 min, 72°C 1 min (35 cycles), and 72°C 15 min
DNApol	PolR-5′cccacctcgggctc aaacac