Molecular changes and immunohistochemical (IHC) expression of p53 and Smad4 in the five studied Peutz-Jeghers syndrome related carcinomas
| Patient with STK11/LKB1 germline mutation | 19p LOH | 5q LOH/APC mutational analysis (DGGE) | K-ras codon 12 mutation | 18q LOH/IHC Smad 4 expression | 17p LOH/IHC p53 overexpression/p53 mutation exons 5–8 | |
|---|---|---|---|---|---|---|
| *Fragment S4 from the APC mutation cluster region is non-informative. | ||||||
| DGGE, denaturing gradient gel electrophoresis; LOH, loss of heterozygosity. | ||||||
| Stomach carcinoma | Unknown | + | −/+ | − | −/+ | −/−/− |
| Small bowel carcinoma | + | + | −/−* | − | −/+ | −/+/− |
| Pancreas carcinoma | + | + | −/+ | − | −/+ | −/−/− |
| Colon carcinoma | + | + | −/+ | − | +/+ | −/−/− |
| Colon carcinoma (fresh frozen) | Unknown | + | −/+ (truncationof segment 2) | + | −/+ | −/−/− |