Antibody characteristics
| Antibody/antigen | Type | Source | Dilution | Incubation | Pretreatment | Cell pattern |
|---|---|---|---|---|---|---|
| CEA, carcinoembryonic antigen; M, monoclonal mouse; P, polyclonal rabbit; RT, room temperature; SP-A, surfactant apoprotein A; TTF-1, thyroid transcription factor 1. | ||||||
| Calretinin | P | Zymed (San Francisco, USA) | 1/50 | Overnight/4° C | Hotplate | Cytoplasm |
| CEA | M | Dako (Ely, UK) | 1/20 | 30–60 min/RT | Microwave | Cytoplasm |
| Cytokeratin 5/6 | M | Dako | 1/50 | 30–60 min/RT | Microwave | Pericellular and cytoplasm |
| BerEP4 | M | Dako | 1/50 | 30–60 min/RT | Pronase | Membrane |
| E-cadherin | M | Zymed | 1/25 | Overnight/4° C | Hotplate | Membrane |
| HBME-1 | M | Dako | 1/100 | 30–60 min/RT | No pretreatment | Membrane and cytoplasm |
| LeuMI (CD15) | M | Dako | 1/40 | Overnight/4° C | Microwave | Cytoplasm |
| N-cadherin | M | Zymed | 1/25 | Overnight/4° C | Hotplate | Cytoplasm |
| SP-A | P | Chemicon (Harrow, UK) | 1/50 | Overnight/4° C | Hotplate | Membrane and cytoplasm |
| Thrombomodulin | M | Dako | 1/100 | Overnight/4° C | No pretreatment | Membrane |
| TTF-1 | M | Lab Vision (Newmarket, UK) | 1/50 | 30–60 min/RT | Microwave | Nucleus |