NADPH subunits detected by various techniques in human experiments
| Subunit | Cell type | Western blotting | Northern blotting | RT-PCR | ICH | DNA sequencing | Spectroscopy | Anti-sense | Refs |
|---|---|---|---|---|---|---|---|---|---|
| Cell type: ca ec, coronary artery endothelial cell; fb, fibroblast; huvec, human umbilical vein endothelial cell; vsmc, vascular smooth muscle cell. | |||||||||
| ICH, immunohistochemistry; RT-PCR, reverse transcription polymerase chain reaction. | |||||||||
| p22phox | huvec | + | + | + | + | 23–25 | |||
| − | 23 | ||||||||
| ca ec | + | + | 26 | ||||||
| vsmc | + | + | + | + | + | 16, 26, 27 | |||
| fb | + | + | 23, 28 | ||||||
| gp91phox | huvec | + | + | 23–25 | |||||
| − | 23 | ||||||||
| vsmc | − | − | 24 | ||||||
| fb | |||||||||
| p47phox | huvec | + | + | + | 23, 25 | ||||
| vsmc | + | + | 29 | ||||||
| fb | |||||||||
| p67phox | huvec | + | + | + | 23, 25 | ||||
| vsmc | − | − | 29 | ||||||
| fb | |||||||||