Primers used in PCR amplification of human papillomavirus DNA showing the corresponding annealing temperatures and PCR product sizes
| Primers | Annealing temperature | PCR product |
|---|---|---|
| M, A+C; R, A+G; W, A+T; Y, C+T. PCR was first performed using the degenerate MY09/MY11 primer set, following by nested PCR with the GP5+/GP6+ set. | ||
| MY09: 5′-CGTCCMARRGGAWACTGATC-3′ | 55°C | 450 bp |
| MY11: 5′-GCMCAGGGWCATAAYAATGG-3′ | 55°C | 450 bp |
| GP5+: 5′-TTTGTTACTGTGGTAGATAC TAC-3′ | 40°C | 150 bp |
| GP6+: 3′-CTTAT ACTAAATGTCAAATAAAAAG-5′ | 40°C | 150 bp |