Fixation and processing protocols for submitting laboratories
| Hospital processing equipment | Fixative | Reagent | C | T | V/P | TD | Reagent | C | T | V/P | TD | Reagent | T | V/P | C | TD |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fixation and processing protocols for laboratories submitting paraffin embedded blocks for ER assay. | ||||||||||||||||
| C, number of changes of reagent; T, temperature in degrees C; V/P, vacuum/pressure; TD, total duration of stage of processing (minutes); FS, formal saline; NBFS, neutral buffered formal saline; IPA, isopropyl alcohol. | ||||||||||||||||
| 1 Bayer VIP 2000 | 10% FS | Alcohol | 7 | 37 | +/+ | 370 | Xylene | 3 | 37 | +/+ | 120 | Wax | 60 | +/+ | 4 | 285 |
| 2 Leica TP 1050 | 10% NBFS | Industrial methylated spirit | 5 | A | +/+ | 300 | Xylene | 4 | A | +/+ | 180 | Wax | 60 | +/+ | 4 | 360 |
| 3 Shandon hypercenter XP | 10% FS | Alcohol | 6 | A | −/− | 390 | IPA/chloroform 50/50 | 1 | A | −/− | 30 | Wax | 60 | +/+ | 2 | 300 |
| Paraffin wx | 2 | A | +/+ | 120 | ||||||||||||
| 4 Leica TP1050 | 10% FS | Industrial methylated spirit | 6 | 40 | +/+ | 360 | Xylene | 3 | 40 | +/+ | 180 | Wax | 56 | +/+ | 3 | 240 |
| 5 Shandon Pathcentre | 10% NBFS | IPA | 6 | A | +/+ | 420 | Chloroform | 2 | A | +/+ | 180 | Wax | 56 | +/+ | 4 | 360 |
| Chloroform/xylene (1/1) | 1 | A | 90 | |||||||||||||
| 6 Shandon Pathcentre | 10% NBFS | IPA | 7 | A | +/+ | 540 | Chloroform | 3 | A | +/+ | 180 | Wax | 60 | +/+ | 4 | 180 |
| 7 Shandon hypercenter XP | 10% acetic FS | Alcohol | 8 | A | +/+ | 480 | Xylene | 2 | 37 | +/+ | 180 | Wax | 60 | +/+ | 2 | 240 |