Table 1

IgA tTG commercial enzyme linked immunosorbent assay kits: manufacturers' kit details

ManufacturerSource of tTGCalcium activated tTGSerum dilutionIncubation times (serum, conjugate) in minutesConjugateSubstrate
†In the manufacturer's kit insert, alkaline phosphatase was mentioned under “Principle”, but HRP was mentioned under “Reagent supplied”.
HRP, horseradish peroxidase; NS, not stated; PNPP, paranitrophenyl phosphate; TMB, 3,3`,5,5` tetramethylbenzidine; tTG, tissue transglutaminase.
AESKULISA/Aesku.Lab Diagnostica (Wendelsheim, Germany)Recombinant humanNS1/10030, 15HRP antihuman IgATMB
The Binding Site (Birmingham, UK)Guinea pig liverYes1/10030, 30Rabbit HRP antihuman IgATMB
Recombinant humanNo1/10030, 30Rabbit HRP antihuman IgATMB
Eurospital S.p.A (Trieste, Italy)Guinea pig liverNS1/2560, 60Sheep HRP antihuman IgATMB
Recombinant humanNS1/2560, 60Sheep HRP antihuman IgATMB
Genesis Diagnostics (Littleport, UK)Guinea pig liverYes1/10030, 30Rabbit HRP antihuman IgATMB
ImmuLisa/Immco Diagnostics Inc (Buffalo, New York, USA)Guinea pig liverNS1/5060, 30Alkaline phosphatase antihuman IgAPNPP
Immunopharmacology Research Diagnostics (Catania, Italy)Guinea pig liverNS1/10030, 30HRP antihuman IgA†TMB
QUANTA Lite/Inova (Diagnostics Inc, San Diego, California, USA)Guinea pig liverNS1/10030, 30Goat HRP antihuman IgATMB
Purified human erythrocyteNS1/10030, 30Goat HRP antihuman IgATMB
Medizyme/Medipan Diagnostica GmbH (Selchow, Germany)Guinea pig liverNS1/5060, 30Sheep HRP antihuman IgATMB
Orgentec Diagnostika GmbH (Mainz, Germany)Purified humanNS1/10030,15Rabbit HRP antihuman IgATMB
Varelisa/Pharmacia & Upjohn Diagnostics GmbH & Co (Freiburg, Germany)Recombinant humanNS1/10030, 30HRP antihuman IgATMB