IgA EMA and IgA tTG results in patients with CD and controls using the manufacturers' cut off points and ROC plot analysis derived decision thresholds for the seven guinea pig liver tTG based ELISA kits
| Manufacturer's cut off point | ROC plot analysis | |||||||
|---|---|---|---|---|---|---|---|---|
| Assay type/Manufacturer | Cut off | CD (sensitivity) | Non-CD controls (specificity) | IBD controls | Threshold | CD (sensitivity) | Non-CD controls (specificity) | IBD controls |
| Results equal to or greater than the cut off/threshold were considered positive. Specificity was calculated using only the non-CD controls (see text). | ||||||||
| AU, arbitrary units; CD, coeliac disease; ELISA, enzyme linked immunosorbent assay; IBD, inflammatory bowel disease; IgA EMA, IgA anti-endomysial antibody; IgA tTG, IgA anti-tissue transglutaminase antibody; IIF, indirect immunofluorescence; NA, not applicable; tTG, tissue transglutaminase; ROC, receiver operating characteristic. | ||||||||
| IgA EMA IIF/The Binding Site | 1/4 | 49/49 (100%) | 0/34 (100%) | 0/30 | NA | NA | NA | NA |
| Guinea pig liver tTG based ELISA/The Binding Site | 4 U/ml | 43/49 (88%) | 3/34 (91%) | 0/30 | 3.5 U/ml | 43/49 (88%) | 3/34 (91%) | 0/30 |
| Guinea pig liver tTG based ELISA/Eurospital | 5 AU | 48/49 (98%) | 22/34 (35%) | 7/30 | 9 AU | 46/49 (94%) | 4/34 (88%) | 0/30 |
| Guinea pig liver tTG based ELISA/Genesis Diagnostics | 10 U/ml | 47/49 (96%) | 8/34 (76%) | 2/30 | 15.3 U/ml | 44/49 (90%) | 4/34 (88%) | 1/30 |
| Guinea pig liver tTG based ELISA/ImmuLisa | 20 EU/ml | 45/49 (92%) | 8/34 (76%) | 0/30 | 22.2 EU/ml | 44/49 (90%) | 6/34 (82%) | 0/30 |
| Guinea pig liver tTG based ELISA/ Immunopharmacology Research Diagnostics | 25 AU | 49/49 (100%) | 30/34 (12%) | 16/30 | 76.4 AU | 43/49 (88%) | 4/34 (88%) | 2/30 |
| Guinea pig liver tTG based ELISA/QUANTA Lite | 20 units/ml | 42/49 (86%) | 0/34 (100%) | 0/30 | 14.1 units/ml | 45/49 (92%) | 1/34 (97%) | 1/30 |
| Guinea pig liver tTG based ELISA/Medizyme | 25 U/ml | 48/49 (98%) | 16/34 (53%) | 15/30 | 38.5 U/ml | 43/49 (88%) | 4/34 (88%) | 2/30 |