Comparison of diameter and cross sectional area of type 1 and 2 muscle fibres in ATPase and immunohistochemistry (IHC) methods
| Staining method | Type 1 | Type 2 (2a and 2b) | Type 2a | Type 2b | All |
|---|---|---|---|---|---|
| IHC | |||||
| No. of fibres measured | 733 | 420 | 447 | 1600 | |
| Mean minor axis/μm | 64.8 | 61.0 | 65.5 | 64.0 | |
| SD of minor axis/μm | 11.3 | 12.6 | 13.0 | 12.3 | |
| Mean area/μm2 | 4923 | 4616 | 5227 | 4928 | |
| SD of area/μm2 | 1444 | 1581 | 1692 | 1568 | |
| ATPase at pH 9.4 | |||||
| No. of fibres measured | 763 | 83.6 | 1599 | ||
| Mean minor axis/μm | 65.4 | 65.8 | 65.6 | ||
| SD of minor axis/μm | 11.8 | 12.0 | 11.9 | ||
| Mean area/μm2 | 5007 | 5264 | 5141 | ||
| SD of area/μm2 | 1504 | 1520 | 1517 |