Antibodies and antigen retrieval techniques used
| Antibody/antigen | Dilution | Retrieval | Source |
|---|---|---|---|
| *Monoclonal antibodies produced and kindly given by Dr U Günthert. | |||
| CD44s, CD44 standard isoform; CD44v, CD44 variant isoform. | |||
| CD10 | 1/10 | Pressure cooker, 121°C, 5 minutes | Dako, Glostrup, Denmark |
| CD20 | 1/700 | Microwave oven, 800 W, 10 minutes | Dako |
| bcl-2 | 1/50 | Pressure cooker, 121°C, 5 minutes | Dako |
| bcl-6 | 1/40 | Pressure cooker, 121°C, 5 minutes | Dako |
| CD44s | 1/10 | Microwave oven, 800 W, 60 minutes | * |
| CD44v4 | 1/10 | Microwave oven, 800 W, 60 minutes | * |
| CD44v6 | 1/10 | Microwave oven, 800 W, 60 minutes | * |
| CD44v9 | 1/10 | Microwave oven, 800 W, 60 minutes | * |