Raw data obtained when the sera were tested by immunofluorescence (IFL), in house enzyme linked immunosorbent assay (ELISA), Varelisa, LKM1-ELISA, and radioligand assay (RLA)
| Diagnosis | IFL LKM1 (titre) | In house ELISA (AU/ml; NV <0.16) | Varelisa (AU/ml; NV <0.55) | LKM1- ELISA (AU/ml; NV <10.78) | RLA (cpm; NV <718) |
|---|---|---|---|---|---|
| Cut off points were: absorbance of 0.16 AU/ml for the in house ELISA, of 0.55 AU/ml for the Varelisa, of 10.78 AU/ml for the LKM1-ELISA, and 718 cpm for the RLA. The numbers in bold denote positive results. The numbers in italic indicate positives for the LKM1-ELISA assay when the cutoff point of 18.74 AU/ml (maximum value seen with a normal control) was used. | |||||
| α1ATD, α1 antitrypsin deficiency; AIH1, autoimmune hepatitis type 1; AIH2, autoimmune hepatitis type 2; Alagille, Alagille syndrome; cpm, counts per minute; NV, normal value. | |||||
| AIH2 | 1/80 | 0.37 | 0.78 | 100 | 1430 |
| 1/160 | 0.44 | 0.82 | 108.8 | 1566 | |
| 1/640 | 0.45 | 1.43 | 125 | 1623 | |
| 1/160 | 0.45 | 1.1 | 108.8 | 1331 | |
| 1/320 | 0.47 | 1.1 | 112.5 | 1515 | |
| 1/2560 | 0.61 | 1.44 | 137.5 | 1403 | |
| 1/2560 | 0.66 | 1.42 | 135 | 1649 | |
| 1/10 240 | 0.65 | 1.45 | 137.5 | 1795 | |
| 1/10 240 | 0.65 | 1.45 | 125 | 1775 | |
| 1/2560 | 0.66 | 1.43 | 137.5 | 1775 | |
| 1/2560 | 0.64 | 1.42 | 137.5 | 1841 | |
| 1/1280 | 0.64 | 1.44 | 137.5 | 1738 | |
| 1/160 | 0.6 | 1.41 | 137.5 | 1663 | |
| 1/640 | 0.51 | 1.24 | 125 | 1804 | |
| 1/40 | 0.51 | 1.13 | 125 | 1788 | |
| 1/320 | 0.53 | 0.79 | 100 | 1810 | |
| 1/40 | 0.51 | 0.7 | 100 | 1830 | |
| 1/80 | 0.42 | 0.68 | 100 | 1572 | |
| 1/80 | 0.37 | 0.59 | 95 | 1590 | |
| 1/80 | 0.36 | 0.5 | 93 | 1776 | |
| 1/1280 | 0.35 | 1.43 | 125 | 1834 | |
| 1/40 | 0.45 | 0.79 | 83.7 | 1797 | |
| 1/40 | 0.36 | 0.72 | 75 | 1526 | |
| 1/10 | 0.4 | 0.64 | 92.5 | 1288 | |
| 1/10 | 0.44 | 0.64 | 95 | 1465 | |
| 1/80 | 0.47 | 1.15 | 121.2 | 1677 | |
| 1/640 | 0.4 | 1.33 | 115 | 1562 | |
| 1/80 | 0.47 | 1.01 | 112.5 | 1623 | |
| 1/640 | 0.47 | 1.39 | 117.5 | 1808 | |
| 1/640 | 0.48 | 1.44 | 132.5 | 1343 | |
| AIH1 | Neg | 0.01 | 0.07 | 12.5 | 634 |
| Neg | 0.04 | 0.08 | 7.5 | 706 | |
| Neg | 0.06 | 0.05 | 3.8 | 687 | |
| Neg | 0.02 | 0.05 | 10 | 643 | |
| Neg | 0.05 | 0.06 | 8.8 | 590 | |
| Neg | 0.03 | 0.05 | 8.8 | 441 | |
| Neg | 0.01 | 0.1 | 16.2 | 473 | |
| Neg | 0.02 | 0.12 | 12.5 | 527 | |
| Neg | 0.02 | 0.07 | 17.5 | 572 | |
| Neg | 0.04 | 0.06 | 16.3 | 377 | |
| Neg | 0.05 | 0.05 | 13.5 | 307 | |
| Neg | 0.03 | 0.03 | 5 | 218 | |
| Neg | 0.01 | 0.11 | 15 | 257 | |
| Neg | 0.08 | 0.08 | 5 | 323 | |
| Neg | 0.12 | 0.08 | 35 | 189 | |
| Neg | 0.12 | 0.14 | 7.5 | 333 | |
| Neg | 0.01 | 0.09 | 8.7 | 399 | |
| Neg | 0.04 | 0.61 | 12.5 | 241 | |
| Neg | 0.07 | 0.08 | 12.5 | 225 | |
| Neg | 0.08 | 0.03 | 6.2 | 200 | |
| Neg | 0.05 | 0.08 | 8.7 | 218 | |
| Neg | 0.09 | 0.07 | 6.2 | 255 | |
| Neg | 0.01 | 0.06 | 7.5 | 275 | |
| Neg | 0.1 | 0.05 | 6.2 | 243 | |
| Neg | 0.15 | 0.05 | 7.5 | 264 | |
| Neg | 0.04 | 0.05 | 6.3 | 220 | |
| Neg | 0.11 | 0.04 | 26.2 | 226 | |
| Neg | 0.03 | 0.05 | 3.7 | 255 | |
| Neg | 0.01 | 0.16 | 10 | 271 | |
| Normal | Neg | 0.01 | 0.05 | 10 | 145 |
| Neg | 0.06 | 0.05 | 5 | 276 | |
| Neg | 0.03 | 0.03 | 5 | 229 | |
| Neg | 0.01 | 0.03 | 2.5 | 200 | |
| Neg | 0.02 | 0.03 | 8.7 | 377 | |
| Neg | 0.02 | 0.05 | 2.5 | 232 | |
| Neg | 0.07 | 0.04 | 5 | 261 | |
| Neg | 0.01 | 0.03 | 1.3 | 257 | |
| Neg | 0.02 | 0.05 | 10 | 224 | |
| Neg | 0.02 | 0.04 | 0.5 | 297 | |
| α1ATD | Neg | 0.04 | 0.04 | 0.5 | 242 |
| Neg | 0.01 | 0.04 | 7.5 | 244 | |
| Neg | 0.07 | 0.15 | 13.7 | 135 | |
| Neg | 0.06 | 0.04 | 5 | 263 | |
| Neg | 0.03 | 0.05 | 8.7 | 236 | |
| Neg | 0.03 | 0.11 | 2.5 | 232 | |
| Neg | 0.06 | 0.09 | 10 | 250 | |
| Neg | 0.05 | 0.05 | 6.2 | 254 | |
| Alagille | Neg | 0.01 | 0.05 | 12.5 | 286 |
| Neg | 0.04 | 0.04 | 3.7 | 246 | |
| Neg | 0.01 | 0.05 | 2.5 | 237 | |
| Neg | 0.03 | 0.05 | 10 | 220 | |
| Neg | 0.07 | 0.1 | 13.5 | 280 | |
| Neg | 0.07 | 0.18 | 7.5 | 275 | |
| Neg | 0.09 | 0.03 | 5 | 261 | |
| Neg | 0.01 | 0.06 | 6.2 | 254 | |