Sequences of primers used
| Primers | Region | Nucleotide sequence |
|---|---|---|
| Primers for CMV are based on the sequence published by Kouzarades et al,24 and for C pneumoniae on that by Campbell et al.25 | ||
| CMV, cytomegalovirus; PCR, polymerase chain reaction. | ||
| CMV | ||
| Outer PCR | ||
| CMVR-1 | IE exon 4 | GTAATGAAGCGCCGCATTGAGGAGA |
| CMVL-1 | IE exon 4 | GGCAACTTCCTCTATCTCAGACAC |
| Inner PCR | ||
| CMVR-2 | IE exon 4 | CCTAGTGTGGATGACCTACGGGCCA |
| CMVL-2 | IE exon 4 | CAGACACAGTGTCCTCCCGCTCCTC |
| Chlamydia pneumoniae | ||
| HL-1 | GTTGTTCATGAAGGCCTACT | |
| HR-1 | TGCATAACCTACGGTGTGTT | |
| β actin | ||
| β3 | TGGACTTCGAGCAAGAGATG | |
| β4 | CTCCTTCTGCATCCTGTC | |