Antigen | Clone | Source | Antigen retrieval | Dilution | Incubation time |
---|---|---|---|---|---|
CDK4, cyclin dependent kinase 4; mdm2, murine double minute 2; MW, citric acid in microwave; pRb, retinoblastoma protein; WB, citric acid in water bath. | |||||
Cyclin D1 | DCS-6 | Dako | WB | 1/100 | 60 minutes |
CDK4 | C-22 | Santa Cruz | – | 1:30 | 60 minutes |
p16 | Ab-4 JC2 | Neomarkers | WB | 1:75 | 60 minutes |
pRb | RB-1 | Dako | WB | 1:1000 | 45 minutes |
E2F-1 | KH95 | Santa Cruz | MW | 1:1500 | 45 minutes |
p53 | DO-7 | Dako | WB | 1:75 | 40 minutes |
mdm2 | 1B10 | Novocastra | WB | 1:50 | 40 minutes |
c-erbB-2 | Polyclonal | Dako | WB | 1:100 | 40 minutes |
Bcl-2 | bcl-2/100/D5 | Novocastra | MW/EDTA | 1:150 | 40 minutes |