Fas | Paraffin wax | MW 3×10 min at 98°C in 1mM EDTA; pH 8.0 | Mouse monoclonal at 1/400 dilution; Upstate Biotechnology, Lake Placid, USA |
Bcl-xl | Paraffin wax | MW 2×15 min at 98°C in 0.1M Tris HCl; pH 9.0 | Mouse monoclonal at 1/100 dilution; Zymed Laboratories, South San Francisco, California, USA |
Bax | Paraffin wax | MW 1×8 min boiling in 10mM citrate; pH 6.0 | Mouse monoclonal at 1/400 dilution; Santa Cruz Biotechnology P19, Santa Cruz, California, USA |
Bcl-2 | Paraffin wax | MW 1×8 min boiling in 10mM citrate; pH 6.0 | Mouse monoclonal at 1/50 dilution; Dako, Glostrup, Denmark |
COX-2 | Paraffin wax | MW 3×10 min at 98°C in 1mM EDTA; pH 8.0 | Mouse monoclonal at 1/50 dilution; Transduction Laboratories, Lexington, Kentucky, USA |
iNOS | Paraffin wax | MW 3×10 min at 98°C in 1mM EDTA; pH 8.0 | Mouse monoclonal at 1/50 dilution; Transduction Laboratories |
Active caspase 3 | Paraffin wax | MW; 1×8 min in 10mM citrate buffer; pH 6.0 | Rabbit polyclonal at 1/100 dilution; Cell Signalling Technology, Beverly Massachusetts, USA |
Ki-67 | Paraffin wax | 3×15 min at 115°C in 0.2% SDS in maleate buffer; pH 6.0 | Mouse monoclonal MIB-1 at 1/400 dilution; Immunotech, Marseille, France |