Oligonucleotide primer and probe sequences used
| Gene | Oligonucleotide | Sequence | PCR product size (bp) |
|---|---|---|---|
| GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PEDF, pigment epithelium derived factor. | |||
| PEDF | Upper primer | 5′-AGGCCCAGAGTCCTGACGGG-3′ | 151 |
| Lower primer | 5′-CCTTGAAGTGCGCCACACCG-3′ | ||
| Probe | 5′-CGCAGATGAAAGGGAAGCTCGCCAGGT-3′ | ||
| GAPDH | Upper primer | 5′-GAAGGTGAAGGTCGGAGTCA-3′ | 226 |
| Lower primer | 5′-GAAGATGGTGGTGATGGGATTTC-3′ | ||
| Probe | 5′-CAAGCTTCCCGTTCTCAGCC-3′ | ||