Oligonucleotide primers used to amplify the 16S rRNA and Helicobacter pylori specific genes
| Gene | Sequence (5′ to 3′) | Reaction conditions | Amplicon size (bp) | Position |
|---|---|---|---|---|
| 16S rRNA | GTCATGACGGGTATCC ACTTCACCCCAGTCGCTG | 94°C for 1 min, 55°C for 2 min, 72°C for 2.5 min (35 cycles) | 1200 bp | 276–291 1495–1478 |
| 98°C for 10 min (1 cycle) | ||||
| 26 kDa | TGGCGTGTCTATTGACAGCGAGC | 94°C for 30 sec, 68°C for 1 min (37 cycles) | 298 bp | 474–496 |
| CCTGCTGGGCATACTTCACCATG | 94°C for 30 sec, 68°C for 1 min, 72°C for 2 min (6 cycles) | 776–754 | ||
| cagA | ATAATGCTAAATTAGACAACTTGAGCGA TTAGAATAATCAACAAACATCACGCCAT | 94°C for 1 min, 55°C for 1 min, 72°C for 1.5 min (35 cycles) | 297 bp | 1751–1778 2048–2021 |
| glmM | GGATAAGCTTTTAGGGGTGTTAGGGG GCTTACTTTCTAACACTAACGCGC | 94°C for 1 min, 56°C for 1 min, 72°C for 1 min (35 cycles) | 296 bp | 1289–1314 1584–1561 |
| vacA | GGAGCCCCAGGAAACATTG CATAACTAGCGCCTTGCAC | 94°C for 1 min, 52°C for 1 min, 72°C for 1 min (35 cycles) | 352 bp | 976–994 1327–1309 |
| rps4 | ATGAGTCTTTCTTCATATGGGA ATGGCAAGATATAGAGGTGCAG | 94°C for 1 min, 58°C for 1 min, 72°C for 1 min (40 cycles) | 605 bp | 605–584 1–22 |