Profiles of antibodies used in our study
| Antigen/antibody (dilution) | Source | Pretreatment |
|---|---|---|
| *Polyclonal antibody. | ||
| EGFR, epidermal growth factor receptor; ER, oestrogen receptor; PgR, progesterone receptor; SMA, smooth muscle actin. | ||
| S-100 protein (1/2000)* | DakoCytomation, Glostrup, Denmark | None |
| SMA (1A4; 1/100) | DakoCytomation | Autoclave |
| Keratin 14 (LL002; 1/50) | Ylem, Rome, Italy | Autoclave |
| EGFR (2-18C9; prediluted) | DakoCytomation | Proteinase K |
| HER2/neu (1/100)* | DakoCytomation | Autoclave |
| ER (1D5; 1/50) | DakoCytomation | Autoclave |
| PgR (1A6; 1:50) | Novocastra, Newcastle upon Tyne, UK | Autoclave |
| p53 (DO7; 1/100) | DakoCytomation | Autoclave |