CK20 | Nested RT-PCR | Two 35 cycle reactions | 104 ZR75.1 or 105 MCF-7/T47-D cells detected in 107 CK20 negative PBPCs or 1–100 ng of total tumour RNA, depending upon tumour or cell line | Not specific | 30 |
c-erbB-2 | Semi-nested RT-PCR | One 30 cycle reaction and one 20 cycle reaction | 1 MCF-7 cell in 106 CCRF-CEM cells | Not specific | 52 |
Maspin | Nested RT-PCR | Two 35 cycle reactions | 1 MCF-7 cell in 106 PBMN | NA | 53 |
β-hCG | RT-PCR | One 30 cycle reaction | 1 BC cell in 107 PBL | Specific | 54 |
EGFR | Semi-nested RT-PCR, Southern blot | One 30 and one 35 cycle reaction | Detect EGFR transcripts in 30 fg MDA-MB-468 cell RNA | Not specific | 55 |
PTHRP | Nested RT-PCR | Two 30 cycle reactions | 1 786 cell in 106 MNC | Specific | 56 |
MUC1 | Q RT-PCR | One 45 cycle reaction | Detect 5 pg of MCF7 RNA or 1 MUC1 expressing cell in 5 ml blood | Not specific | 57 |
CK19 | Nested RT-PCR | Two 40 cycle reactions | 1 MCF-7 cell detected in 106 BM cells | Specific | 27 |
CK19 | Nested RT-PCR | Two 35 cycle reactions | ND | NA | 58 |
CK19 | Nested RT-PCR | Two 35 cycle reactions | 10 T47-D cells in 106 PBMN | Specific | 59 |
CK19 | Nested RT-PCR | Two 35 cycle reactions | 2 BC cells detected in 106 cells or a 10−6 dilution of cDNA reverse transcribed from 1–2 µg BC RNA | Not specific | 60 |
CK19 | Competitive nested RT-PCR | Two 30 cycle reactions | 10−6/10−7 dilutions of MCF-7 cells in CK19 negative PBL | Not specific | 61 |
CK19 | Nested RT-PCR | One 20 cycle reaction and one 22 cycle reaction | <1 MCF-7 cell in 106 PBL | Specific | 62 |
CK19 | Q RT-PCR | One 50 cycle reaction | 1 CK19 positive cells in 106 negative cells | Not specific | 63 |
CK19 | Nested RT-PCR | Two 35 cycle reactions | 1 MCF-7 cell in 106 PBMN | Not specific | 64 |
CK19 | Nested RT-PCR | Two 35 cycle reactions | ND | NA | 65 |
CK19 | Q RT-PCR | One 50 cycle reaction | 1 MCF-7 cell equivalent | Not specific | 66 |
Mammaglobin | Nested RT-PCR | Two 35 cycle reactions | 1 pg T47-D RNA or >100 ng MCF-7/ZR75.1 RNA in 1 µg RNA mixture or 1 T47D cell in 106 or 1 MCF-7/ZR75.1 cell in 10 haemopoietic control cells | Specific | 67 |
Mammaglobin | Nested RT-PCR | Two 30 cycle reactions | 1 SKBR5 BC cell in 106–107 WBC | Specific | 68 |
Mammaglobin | Q RT-PCR | One 45 cycle reaction | 1 EFM-192 BC cell in 1 ml blood | Specific | 69 |
Mammaglobin | Nested RT-PCR | Two 40 cycle reactions | ND | Specific | 70 |
Mammaglobin | RT-PCR | One 35 cycle reaction | ND | ND | 71 |
Her2/neu | Semi-nested RT-PCR | Two 20 cycle reactions | Detect 3 pg MCF-7 RNA in 3 μg Raji cell line RNA | Specific | 72 |