Details of the antibodies, dilutions, and antigen retrieval methods used
| Primary antibody | Dilution/incubation time | Antigen retrieval | Specificity | Source |
|---|---|---|---|---|
| *At room temperature; †slides are placed in antigen unmasking buffer pH 6.0 (TAR001; YLEM) then microwaved (3×4 minutes); ‡predigestion with protease type XXIV for 20 minutes at 37°C. | ||||
| HIF1α, hypoxia inducible factor-1α; LDH5, lactate dehydrogenase-5; TP, thymidine phosphorylase; VEGF, vascular endothelial growth factor. | ||||
| Ki-67 | 1/75 (75 min*) | MW† | MIB1 | YLEM, Avezzano, Italy |
| P-GF.44C | 1/4 (75 min*) | MW† | TP | Oxford University, Oxford, UK |
| VG1 | 1/4 (75 min*) | MW† | VEGF | Oxford University |
| JC70 (CD31) | 1/20 (30 min*) | Protease‡ | Endothelium | Dako, Glostrup, Denmark |
| ESEE 122 | 1/20 (overnight) | MW† | HIF1α | Oxford University |
| 9002 | 1/100 (75 min*) | MW† | LDH5 | Abcam, Cambridge, UK |