Selected examples of phage antibodies with applications in clinical settings
| scFv target | Clinical use | Type of selection | Ref |
|---|---|---|---|
| CEA, carcinoembryonic antigen; EGFR, endothelial growth factor receptor; HIV, human immunodeficiency virus; RBC, red blood cell; scFV, single chain heavy and light chain variable region; VEGF, vascular endothelial growth factor. | |||
| scFvs genetically engineered from existing antibodies | |||
| Novel tumour associated antigen | Tumour imaging (melanoma) | scFv of H11 murine monoclonal antibody | 30 |
| Anti-idiotypic antibody of ACA125 | Tumour targeting (ovarian) | scFv of murine antibody ACA125 | 31 |
| Tumour associated glycoprotein-72 | Tumour targeting (colon) | scFv of CC49 murine monoclonal antibody | 32 |
| Platelet glycoprotein 1bα | Anti-thrombotic agent | scFv of SZ-2 murine monoclonal antibody | 33 |
| Murine scFvs that have undergone affinity enrichment by phage display | |||
| HMW-MAA | Tumour targeting (melanoma) | Affinity enrichment of murine scFv | 34 |
| CD30 | Tumour targeting (Hodgkin) | Affinity enrichment of Ki4 murine scFv | 25 |
| EpCAM | Tumour targeting (colon) | Affinity enrichment of the MOC-31 murine antibody | 35 |
| L1 | Role of L1 in nervous system | Affinity enrichment of murine antibody | 36 |
| scFvs selected from immunised phage libraries | |||
| CEA | Tumour targeting (colon) | Selected from a CEA immunised mouse hybridoma library | 20 |
| EGFR | Tumour targeting (general) | Selected from an EGFR immunised mouse hybridoma library | 22 |
| Pertussis toxin | Neutralisation of pertussis infection | Selected from a library assembled from patients recovering from pertussis infection | 37 |
| p24 (HIV) | HIV diagnosis | Selected from a viral lysate immunised mouse hybridoma library | 38 |
| Human scFvs selected from naïve phage libraries | |||
| CD55 | Tumour targeting (lung) | Naïve library selected on lung carcinoma cell line | 39 |
| EpCAM | Tumour targeting (colon) | Naïve library selected on colorectal cell line | 40 |
| Fibronectin | Tumour targeting | Naïve library selected on fibronectin fusion protein | 41 |
| VEGF | Blocking angiogenesis | Naïve library selected on recombinant VEGF | 42 |
| Thrombospondin-1 | Sickle RBC adhesion | Naïve library selected on thrombospondin-1 | 29 |