Comparison of immunohistochemistry and western blot results in the index and control placentas
| Method | Index placenta | Control placentas |
|---|---|---|
| Akt, protein kinase B; AP, alkaline phosphatase; GSK-3β, glycogen synthase kinase-3β; p38-MAPK, p38 mitogen activated protein kinase; Erk1/2, extracellular signal regulated kinase1/2; SAPK/JNK, stress activated protein kinase/c-Jun N-terminal kinase. | ||
| Immunohistochemistry | Slight and discontinuous AP staining in syncytiotrophoblast brush border membrane | Intensive continuous AP staining in syncytiotrophoblast brush-border membrane |
| 8–10% Ki-67 positive syncytiotrophoblast cells/field on average | 1–2% Ki-67 positive syncytiotrophoblast cells/field on average | |
| Western blot | Total-Akt 103% | Total-Akt 100% |
| Total-GSK-3β 106% | Total-GSK-3β 100% | |
| Phospho-Akt 174% | Phospho-Akt 100% | |
| Phospho-GSK-3β 152% | Phospho-GSK-3β 100% | |
| Phospho-Erk 1/2 561% | Phospho-Erk 1/2 100% | |
| Phospho-SAPK/JNK 202% | Phospho-SAPK/JNK 100% | |
| p38MAPK 249% | p38MAPK 100% | |