Details of the antibodies, dilutions, and antigen retrieval methods used
| Primary antibody | Dilution (incubation time) | Antigen retrieval | Specificity | Source |
|---|---|---|---|---|
| Incubation with the primary antibodies was at room temperature. | ||||
| EGFR, endothelial growth factor receptor; ER, oestrogen receptor; MW, microwave heating; PR, progesterone receptor. | ||||
| ID5 | 1/20 (75 min) | MW | ER | Immunon-Shandon, Pittsburgh, Pennsylvania, USA |
| IA6 | 1/20 (75 min) | MW | PR | Immunon-Shandon |
| H11 | 1/200 (75 min) | MW | EGFR | Dako, Carpinteria, California, USA |
| MIB-1 | 1/75 (75 min) | MW | Ki-67 | Dako, Glostrup, Denmark |
| JC70 (CD31) | 1/20 (30 min) | Protease | Endothelium | Dako, Glostrup, Denmark |