Primer sequences and polymerase chain reaction conditions for sequencing of 13 exons and exon–intron boundaries of STRAD
| Exon | Forward primer | Reverse primer | Tm (°C) | MgCl2 (mM) |
|---|---|---|---|---|
| 1 | ggtctggcggcggcgcggcagtaaaac | cagtccctcctggctctgccaccgattccatctcc | 65 | 1.25 |
| 2 | ataggtgaagtccagtgctttgaatgg | ctgaaaagcagtgtattttggca | 52 | 1.25 |
| 3 | gtctaaaggctgatagctaatttgttgcggc | caatctatagcaattggcaattctaagggcaagtgtaacc | 65 | 2.5 |
| 4 | ttgctgttctgaatgtgggtgtgtcttctg | ggctgttgcatggagatgacttcttgtggaagtttgtgtg | 52 | 1.25 |
| 5 | cctcgtgggtctgaattaccaccttcacccca | ccaggggctgctgcagctttctcagcag | 65 | 1.25 |
| 6 | ccttttgttgttcttgtccttgcgtatggc | gagtgtgtcacatggagggaggg | 65 | 1.25 |
| 7+8 | gctgccatctttgtgttgagcggc | ggcggcctttcccatgattacag | 65 | 1.25 |
| 9 | gccaggtggagtgtgtgaagacca | gggagccttcaggcattgcc | 65 | 1.25 |
| 10 | ctctctttagttctaccc | gagtagctctcacactcagg | 52 | 2.5 |
| 11 | ccgctacagcccaggtcc | cttcgggagagcagagggtg | 65 | 2.5 |
| 12+13 | ggctgaggcataagtggcagga | ggatgcagaggccacccagag | 65 | 1.25 |