The killing action of UMFix on herpes simplex virus and its effect on nucleic acid detection by real time PCR
| HSV diluent (duplicate test) | Viability after exposure for 20 minutes | Real time PCR Ct* |
|---|---|---|
| *Ct, cycle at which there is a significant increase in fluorescence, which is associated with an exponential growth of PCR product during the log linear phase. | ||
| HSV, herpes simplex virus; PCR, polymerase chain reaction. | ||
| UMFix-1 | No growth | 30.7 |
| UMFix-2 | No growth | 30.7 |
| Saline-1 | Growth | 30.8 |
| Saline-2 | Growth | 31.7 |
| HSV (original inoculum) | Growth | 20.5 |