Table 1

Sequence of primers and PCR conditions for DNA analysis

Gene	Primer sequence 5′–3′	Length of product	Ta	Cycles
EGFR, epidermal growth factor receptor; PCR, polymerase chain reaction; Ta, annealing temperature; TTR, transthyretin.
EGFR	Forward: GAA GCC AAG CCA AAT GGC A	90 bp	60	45
	Reverse: GCT CCA ATA AAT TCA CTG CT
p16	Forward: AGC TTC CTT TCC GTC ATG C	204 bp	60	45
	Reverse: 5-GCA GCA CCA CCA GCG TG
TTR1	Forward: CAG CAG GTT TGC AGT CAG AT	291 bp	60	45
	Reverse: GGT ACC CTT GCC CTA GTA AT
TTR2	Forward: TGG TGG AAA TGG ATC TGT CTG	333 bp	60	45
	Reverse: TGG AAG GGA CAA TAA GGG AAT