Immunoreactivity of markers showing discrepant staining according to the fixative used
| Antigen | Location | Bouin’s | Formalin | ||
|---|---|---|---|---|---|
| Number of slides (cases) | Results | Number of slides (cases) | Results | ||
| Good: the staining pattern was consistent with the expectation based on previous reports; decreased: for p27, a lower percentage of staining was seen, with persistent cytoplasmic staining; aberrant: for C-erb-B2, an altered pattern of spatial expression was noted—although the staining was crisp, the location of the staining was aberrant. | |||||
| MCM2 | Nuclear | 6 (5) | Negative | 22 (11) | Good |
| p27 | Nuclear | 109 (23) | Decreased | 10 (5) | Good |
| C-erb-B2 | Membrane | 23 (4) | Aberrant | 15 (7) | Good |