Expression and imprinting of DLK1, and histology of 30 Wilms tumours
| DLK1 gene | Histology | Myogenesis | |||
|---|---|---|---|---|---|
| Q-PCR | Genotype | Allelic expression | |||
| Bl, blastemal predominant; Epi, epithelial predominant; LOH, loss of heterozygosity; LOI, loss of imprinting; NA, not available; NI, not informative; Q-PCR, quantitative real time polymerase chain reaction; Str, stromal predominant; Tri, triphasic; absent –, low +, moderate ++, high +++. | |||||
| IGF2 LOI tumours | |||||
| LOI-1 | 0.02 | T/C | C/– | Epi | – |
| LOI-2 | 0.1 | T/C | T/– | Bl | – |
| LOI-3 | 0.1 | T/C | C/– | Bl | – |
| LOI-4 | 0.1 | T/C | T/– | Bl | – |
| LOI-5 | NA | T/C | C/– | Tri | ++ |
| LOI-6 | 0.9 | T | NI | Bl | – |
| LOI-7 | 0.1 | T | NI | Bl | – |
| LOI-8 | NA | C | NI | Bl | – |
| LOI-9 | 8.2 | C | NI | Bl/Epi | – |
| LOI-10 | 0.2 | C | NI | Bl | – |
| LOI-11 | 41.6 | C | NI | Epi/Bl | – |
| LOI-12 | 0.4 | T | NI | Bl | – |
| LOI-13 | 0.5 | C | NI | Tri | + |
| LOI-14 | 0.1 | T | NI | Bl | – |
| LOI-15 | 5.6 | C | NI | Tri | ++ |
| LOI-16 | 2.3 | C | NI | Tri | ++ |
| LOI-17 | NA | T | NI | Bl | – |
| WT1 mutant tumours | |||||
| WT1–1 | 4.5 | T/C | T/– | Str | ++ |
| WT1–2 | 15.2 | T/C | T/– | Str | ++ |
| WT1–3 | 9.7 | T/C | C/– | Str | ++ |
| WT1–4 | 0.7 | T | NI | Str | ++ |
| WT1–5 | 14 | C | NI | Str | +++ |
| WT1–6 | 0.4 | C | NI | Str | ++ |
| WT1–7 | 0.4 | C | NI | Str | ++ |
| WT1–8 | 12.7 | T | NI | Str | +++ |
| 11p15.5 LOH tumours | |||||
| LOH-1 | 2.9 | T/C | T/– | Tri | ++ |
| LOH-2 | 2.2 | T/C | T/– | Bl | – |
| LOH-3 | 6.7 | C | NI | Bl/Epi | – |
| LOH-4 | 0.1 | T | NI | Bl | – |
| LOH-5 | 0.5 | C | NI | Bl | – |