Primers and oligoprobes for the methylation sensitive dot blot assay
| Gene | Primer sequences (sense and antisense) | PCR product | CG sites | 3′DIG labelled oligoprobes* |
|---|---|---|---|---|
| *The bold letters represent the 2 CpG sites analysed in the DNA samples. | ||||
| hTERT | 5′-GGGTTATTTTATAGTTTAGGT-3′ | 224 bp | 27 | 5′-TAGTTGCGTTGTCGGGGTTA-3′ |
| 5′-AATCCCCAATCCCTC-3′ | 5′-GTAGTTGTGTTGTTGGGGTTA-3′ | |||
| APC | 5′-GGGGTTAGGGTTAGGTAGG-3′ | 196 bp | 16 | 5′-GATGCGGATTAGGGCGTTTT-3′ |
| 5′-AACTACACCAATACAACCACATA-3′ | 5′-GGATGTGGATTAGGGTGTTTT-3′ | |||
| p16 | 5′-GGGGGAGATTTAATTTGG-3′ | 194 bp | 11 | 5′-TCGGAGGGGGTTTTTTCGTT-3′ |
| 5′-CAACCCCTCCTCTTTCTT-3′ | 5′-GTTGGAGGGGGTTTTTTTGTT-3′ | |||