Summary of antibodies and methods used
| Antigen | Test kit or antibody clone (manufacturer) | Dilution | Incubation time | Antigen retrieval |
|---|---|---|---|---|
| *Glostrup, Denmark; †Freemont, California, USA; ‡Illkirch, France. | ||||
| AR, androgen receptor; EGFR, epithelial growth factor receptor; ER, oestrogen receptor; PR, progesterone receptor. | ||||
| EGFR (Her-1) | EGFRpharmDX™ (Dako*) | Prediluted | 30 minutes | Proteinase K solution for 5 minutes |
| Her-2 | HercepTest™ (Dako*) | Prediluted | 30 minutes | 1/10 epitope retrieval solution (Dako) at 98°C for 40 minutes, then cooling in epitope retrieval solution for 20 minutes |
| Her-3, Ab-8 | Clone SGP1 (Lab Vision†) | 1/50 | 60 minutes | |
| Her-4, Ab-4 | Clone HFR-1 (Lab Vision†) | 1/50 | 30 minutes | |
| AR | Clone AR441 (Dako*) | 1/50 | 30 minutes | |
| ER | Clone 6F11 (Ventana‡) | Prediluted | 30 minutes | Citrate buffer (pH 6.0) in microwave (160 W) for 30 minutes |
| PR | Clone 1A6 (Ventana‡) | Prediluted | 30 minutes | |