ICAM-1 genotyping 5′ nuclease (TaqMan®) primer and probe sequences
| Primer/Probe | Sequence |
|---|---|
| Positions of polymorphisms are marked in lower case. | |
| FAM, reporter gene; ICAM-1, intercellular cell adhesion molecule 1; MGB, minor groove binding probe; NFQ, non-fluorescent quencher; TAMRA, fluorescence quencher labelled probe; VIC, reporter gene. | |
| ICAM-1 +241 Forward | 5′CAGGGGACCGTGGTCTGTT3′ |
| ICAM-1 +241 Reverse | 5′CATAGGTGACTGTGGGGTTCAA3′ |
| ICAM-1 +241 G allele probe | FAM-TGGGAACAGCCcGTC-MGB-NFQ |
| ICAM-1 +241 A allele probe | VIC-TGGGAACAGCCtGTC-MGB-NFQ |
| ICAM-1 +469 Forward | 5′TCGAGATCTTGAGGGCACCTA3′ |
| ICAM-1 +469 Reverse | 5′CGGCTCACTCACAGAGCACAT3′ |
| ICAM-1 +469 G allele probe | FAM-ACGGTCACCTcGCGGGTGA-TAMRA |
| ICAM-1 +469 A allele probe | VIC-CACGGTCACCTtGCGGGTGA-TAMRA |