Schaefer’s fixative for bone marrow trephine biopsies
| Methanol | 480 ml |
| Glucose phosphate buffer (pH 7.4) | 20 ml |
| Neutralised formalin | 250 ml |
| Glucose phosphate buffer (pH 7.4) | |
| Sodium dihydrogen phosphate | 0.96 g |
| Potassium dihydrogen phosphate | 0.17 g |
| Glucose | 3.08 g |
| Distilled water | Up to 100 ml |
| Neutralised formalin | |
| Formalin (∼35% formaldehyde) | 87.5 ml |
| Distilled water | 12.5 ml |
| Calcium carbonate (∼50 g/l) | 5 g |
| EDTA-formaldehyde for overnight decalcification | |
| Disodium EDTA dihydrate | 25 g |
| 4% formaldehyde (∼10% formalin) | Up to 250 ml |