Densities of different inflammatory cells as detected by immunohistochemistry in group 1 and group 2 patients
| No | Grade | Neutrophils | IM | CD3 | CD4 | CD8 | CD20 | CD117 | CD138 | CD163 |
|---|---|---|---|---|---|---|---|---|---|---|
| Group 1 (patients 1–10) were Helicobacter pylori positive and group 2 (patients11–20) were H pylori negative young patients with inflammation of the cardiac mucosa. | ||||||||||
| Grade (grade of general inflammation) scored as follows: 1, mild; 2, moderate; 3, strong. | ||||||||||
| Neutrophils (density of neutrophils (N) and eosinophils (E)) as detected by haematoxylin and eosin staining: +, sparse; ++, moderate. | ||||||||||
| Immunohistochemical staining: CD3 (T cells), CD4 (T helper cells), CD8 (T suppressor cells), CD20 (B cells), CD117 (mast cells), CD138 (plasma cells), and CD163 (macrophages). The density of cell infiltrates was scored as follows: 0, no cells; 1, low density; 2, moderate density; 3, high density. | ||||||||||
| IM, intestinal metaplasia in the cardiac mucosa. | ||||||||||
| 1 | 2 | N+ | – | 1 | 1 | 1 | 1 | 1 | 2 | 1 |
| 2 | 3 | N++ | – | 2 | 2 | 1 | 3 | 1 | 3 | 1 |
| 3 | 3 | N++, E++ | – | 1 | 1 | 1 | 2 | 1 | 3 | 2 |
| 4 | 2 | N+ | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
| 5 | 1 | E+ | – | 1 | 1 | 1 | 2 | 1 | 2 | 2 |
| 6 | 2 | E++ | – | 1 | 1 | 2 | 2 | 1 | 2 | 1 |
| 7 | 2 | N++, E+ | – | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
| 8 | 3 | N++, E++ | – | 2 | 1 | 1 | 2 | 1 | 3 | 2 |
| 9 | 1 | – | – | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
| 10 | 2 | E++ | – | 2 | 1 | 2 | 0 | 1 | 1 | 0 |
| 11 | 2 | E+ | – | 0 | 0 | 1 | 1 | 1 | 2 | 2 |
| 12 | 2 | E+ | + | 1 | 1 | 1 | 0 | 1 | 2 | 1 |
| 13 | 3 | N++, E++ | – | 1 | 1 | 1 | 1 | 1 | 3 | 1 |
| 14 | 2 | N++, E+ | – | 1 | 1 | 1 | 0 | 1 | 2 | 1 |
| 15 | 1 | – | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
| 16 | 1 | E+ | – | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| 17 | 1 | N+, E+ | + | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
| 18 | 3 | E++ | + | 2 | 2 | 2 | 2 | 1 | 3 | 1 |
| 19 | 1 | E+ | + | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
| 20 | 1 | E++ | – | 0 | 0 | 1 | 0 | 1 | 1 | 1 |