Table 1

 Primer sequences used to amplify BRCA1 exon by exon for denaturing gradient gel electrophoresis12

FragmentForward primerReverse primerSize (bp)
*GC clamp sequence (CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCG) is attached to this site.
Exon 2-1ATGATAAAATGAAGTTGTC*ACACTCTAAGATTTTCTGC204
Exon 2-2TTATCTGCTCTTCGCGTTGCTTCCCTAGTATGTAAGGTC*202
Exon 3GCGCGTTGAGCCTCATTTATTTTCACAAAAGCTAATAATGGAGC*185
Exon 5GTATTCTTTCTACAAAAGG*TCCAACCTAGCATGATTAC208
Exon 6GGTTGATAATCACTTGCTG*CACTTGAGTTGCATTCTTG223
Exon 7ATACATAGGGTTTCTCTTG*AGAAGAAAACAAATGGTTT293
Exon 8TTGCTTGACTGTTCTTTACC*ACTTAAAAAACCTGAGACC209
Exon 9CCCTTTTAATTAAGAAAAC*ACTAAATAGGAAAATACCAG191
Exon 10CATTTGACAGTTCTGCATAC*TTCAGTGCCTGTTAAGTTG217
Exon 11-1ATGACAATTCAGTTTTTGAG*TATTACTGGGTTGATGATG147
Exon 11-2AGCTGCTTGTGAATTTTCTG*ATAAACTGCTGTTCTCATGC243
Exon 11-3TTTTTACAAATACTCATGCCAGCTC*TAGGATTCTCTGAGCATGGC313
Exon 11-4TTTTTTTTTTTGTGTGAGAGAAAAGAATGG*CATCTACCTCATTTAGAACG272
Exon 11-5GAATCAAATGCCAAAGTAGC*GGACGCTCTTGTATTATCTG329
Exon 11-6ATTATAGGAGCATTTGTTACTTTTCGAGTGATTCTATTGG*324
Exon 11-7CAAAAGGTGATTCTATTCAG*ATAAGGTGGGCTTAGATTTC275
Exon 11-8CAGGCATATTCATGCGCTTG*GAAAGTATCGCTGTCATGTC281
Exon 11-9GAGTAACAAGCCAAATGAACGGGGTCTTCAGCATTATTAG*242
Exon 11-10TTGTCAATCCTAGCCTTCC*ATTAGTCCCTTGGGGTTTTC324
Exon 11-11AATAAATGTGTGAGTCAGTG*ACATTCCTCTTCTGCATTTC300
Exon 11-12ATTCAAGGTTTCAAAGCGCCGTATATTAACTGTCTGTAC*238
Exon 11-13aAGGAAGAAAATCAAGGAAAG*TAATGAGTCCAGTTTCGTTG223
Exon 11-13bGCCAAATGTAGTATCAAAGGCAGGTGACATTGAATGTTCC*248
Exon 11-13cAAAATCTGCTAGAGGAAAACTCATCACTGGAACCTATTTC*259
Exon 11-13dTAAAGAAGCCAGCTCAAGCCTGAAATCAGATATGGAGAG*325
Exon 11-13eGCAAGAATGTCAAGAAGTAG*CCATCATCTAACAGGTCATC181
Exon 11-13fAGTCATGCATCTCAGGTTTG*ATAAGTTCTCTTCTGAGGAC280
Exon 11-14CTTTCACCCATACACATTTGTGCAGTCATTTAAGCTATTC*277
Exon 11-15GAGTGTCTGTCTAAGAACAC*TATTTGCAGTCAAGTCTTCC221
Exon 11-16GTTTCTTCACAGTGCAGTGAAATAGACTGGGGCAAACAC*296
Exon 12GTCTGCTTTTACATCTGAACC*AATGCAAAGGACACCACACAC221
Exon 13GCGCGATTTCATTTTCTTGGTGCCGGGAAGGAAAGAATTTTGC*305
Exon 14TCAGAACAAAGCAGTAAAG*AAGATGTCAGATACCACAG257
Exon 15-1ATTGGTGGCGATGGTTTTC*CTCCTCCACATCAACAACCT204
Exon 15-2ACTACCCATCTCAAGAGGAGAAATCAAAGTGTTTGTTCC*195
Exon 16-1GACCAGAACTTTGTAATTC*CCAGCAGTATCAGTAGTAT299
Exon 16-2AAAGTTGCAGAATCTGCCCTAAGTCTTAGTCATTAGGG*252
Exon 17GTGCTAGAGGTAACTCATG*CAGCAGATGCAAGGTATTC213
Exon 18ACAGCACTTCCTGATTTGTCTGAGGTGTTAAAGGGAG*222
Exon 19TCTATCTCCGTGAAAAGAGCTGGTTAGTTTGTAACATC*176
Exon 20TGCTCCACTTCCATTGAAG*TTTGTCAACTTGAGGGAGG220
Exon 21CCTCTCTCCATTCCCCTG*AAGGCTGGTGCTGGAACTC182
Exon 22GCCTGGGTTAAGTATGCAG*ATTGTGTCCTCCCTCTCTG210