Amplicon pools created for BRCA1 to increase the throughput of denaturing gradient gel electrophoresis
| Gene | Pool | Exons in amplicon pool |
|---|---|---|
| Each amplicon pool contained three to five amplicons with relatively large melting temperature differences. | ||
| BRCA1 | A | 7,18, 10, 20 |
| B | 9, 6, 8, 23 | |
| C | 5, 2B, 11-3, 16B | |
| D | 14, 11-4, 22, 24 | |
| E | 2A, 11-1, 16A | |
| F | 19, 13, 21 | |
| G | 17, 11-14, 3 | |
| H | 11-15, 15A, 15B | |
| I | 11-16, 11-2, 12 | |
| BRCA1 exon 11 | A | 11-5, 11-8, 11-10 |
| B | 11-13b, 11-13e, 11-13f | |
| C | 11-7, 11-12, 11-13c | |
| D | 11-9, 11-11, 11-13a | |
| E | 11-6, 11-13d | |