Amplicon pools created for BRCA2 to increase the throughput of denaturing gradient gel electrophoresis
| Gene | Pool | Exons in amplicon pool |
|---|---|---|
| Each amplicon pool contained three to five amplicons with relatively large melting temperature differences. | ||
| BRCA2 | A | 11-1, 27-1, 19 |
| B | 23-1, 18-1, 10-4, 11-3 | |
| C | 8, 20, 27-3, 10-5, 21-1 | |
| D | 15-1, 23-2, 26, 10-6 | |
| E | 12, 10-7, 2, 24, 27-2 | |
| F | 5, 6, 11-17, 15-2 | |
| G | 11-16, 9, 27-4, 18-2 | |
| H | 10-1, 10-3, 10-10, 10-8 | |
| I | 25-1, 14-2, 22-1, 25-2 | |
| J | 14-3, 10-9, 22-2 | |
| K | 4, 3, 7 | |
| L | 16, 11-2, 21-2 | |
| M | 13, 17, 14-1, 10-2 | |
| BRCA2 exon 11 | A | 11-4, 11-8, 11-15h |
| B | 11-5,11-10,11-13, 11-15f | |
| C | 11-6, 11-12, 11-15d | |
| D | 11-7, 11-14, 11-15c | |
| E | 11-9, 11-15g, 11-15m | |
| F | 11-15e, 11-15L, 11-15n | |
| G | 11-15a, 11-15i, 11-15j | |
| H | 11-11, 11-15b, 11-15k | |