Primer/probe | Sequence |
---|---|
*6-Carboxyfluorescein; †6-carboxytetramethylrhodamine. | |
SARS-CoV | |
Primers | |
Forward | 5′-AGCTAACGAGTGTGCGCAAGTA-3′ |
Reverse | 5′-TGATGTTCCACCTGGTTTAACATATAGT-3′ |
Dual labelled fluorescent probe | 5′-(FAM*)AGCCGCCACACATGACCATCTCACTTA(TAMRA†)-3′ |
Oligonucleotide for calibration | 5′-AGGTTAGCTAACGAGTGTGCGCAAGTATTAAGTG AGATGGTCATGTGTGGCGGCTCACTATATGTTAAACCAGGT GGAACATCATCCGG-3′ |
GAPDH mRNA | |
Primers | |
Forward | 5′-GAAGGTGAAGGTCGGAGT-3′ |
Reverse | 5′-GAAGATGGTGATGGGATTTC-3′ |
Dual labelled fluorescent probe | 5′-(FAM*)CAAGCTTCCCGTTCTCAGCC(TAMRA†)-3′ |