Immunostaining protocol
| Antibody | Source | Dilution | Pre-treatment (microwave oven, 850 W) |
|---|---|---|---|
| Cyclin A | Novocastra Laboratories (Newcastle upon Tyne, UK) | 1/75 | 2×5 min, 1mM EDTA (pH 8) |
| Cyclin B1 | BioSource International (Camarillo, California, USA) | 1/200 | 5 min + 15 min 350 W, 10mM citrate buffer (pH 6) |
| Cyclin C | Transduction Laboratories (Lexington, Kentucky, USA) | 1/50 | 5 min + 15 min 350 W, 10mM citrate buffer (pH 6) |
| Cyclin D1 | Oncogene Research (San Diego, California, USA) | 1/100 | 2×10 min, 1mM citrate buffer (pH 6) |
| Cyclin D3 | Dako (Carpinteria, California, USA) | 1/50 | 4×5 min, 1mM EDTA (pH 8) |
| Cyclin E | Novocastra Laboratories | 1/75 | 4×5 min, 1mM EDTA (pH 8) |
| Cyclin H | Santa Cruz Biotechnology (Santa Cruz, California, USA) | 1/300 | 5 min + 15 min 350 W, Tris/EDTA (pH 9) |