Antibodies used on bone marrow trephine biopsy specimens at the Hammersmith Hospital
| Antibody | Dilution | Antigen retrieval | Source |
|---|---|---|---|
| EBV, Epstein-Barr virus; EDTA, ethylene diamine tetra acetate; EMA, epithelial membrane antigen, ER, oestrogen receptor; LMP, latent membrane protein; MW, microwave; PCNA, proliferating cell nuclear antigen; PSA, prostate-specific antigen; PR, progesterone receptor. *Rarely performed on bone marrow trephines. Manufacturer details are as follows: Dako (Carpinteria, California, USA); Novocastra (Newcastle upon Tyne, UK); Becton Dickinson (Franklin Lakes, New Jersey, USA); Santa Cruz (Raleigh, North Carolina, USA); Biogenix (Hertfordshire, UK); Sigma (Perth, Western Australia, Australia). | |||
| Glycophorin C | 1:200 | 20 min MW, citrate pH 6.0 | Dako |
| Myeloperoxidase | 1:2000 | No treatment | Dako |
| CD61 | 1:10 | 10 min MW, citrate pH 6.0 | Dako |
| CD42b | 1:500 | 20 min MW, citrate pH 6.0 | Novocastra |
| Mast cell tryptase | 1:3000 | 10 min MW, citrate pH 6.0 | Dako |
| Neutrophil elastase | 1:200 | 10 min Trypsin | Dako |
| Tdt | 1:50 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD117 | 1:400 | 10 min MW, citrate pH 6.0 | Dako |
| CD34 | 1:100 | 20 min MW, citrate pH 6.0 | Dako |
| CD31 | 1:10 | 10 min MW, citrate pH 6.0 | Dako |
| Factor VIII | 1:1000 | 10 min Trypsin | Dako |
| CD1a | 1:200 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD2 | 1:50 | 20 min EDTA, pH 8.0 | Novocastra |
| CD3 | 1:50 | 20 min EDTA, pH 8.0 | Novocastra |
| CD4 | 1:400 | 20 min EDTA, pH 8.0 | Novocastra |
| CD5 | 1:50 | 20 min EDTA, pH 8.0 | Novocastra |
| CD7 | 1:50 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD8 | 1:50 | 20 min EDTA, pH 8.0 | Novocastra |
| CD10 | 1:20 | 20 min EDTA, pH 8.0 | Novocastra |
| CD15 | 1:10 | 20 min MW, citrate pH 6.0 | Dako |
| CD20 | 1:250 | 20 min MW, citrate pH 6.0 | Dako |
| CD21 | 1:50 | 15 min Trypsin | Dako |
| CD23 | 1:100 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD25 | 1:100 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD30 | 1:50 | 20 min Dako retrieval solution, pH 6.0 | Dako |
| CD38 | 1:50 | 20 min EDTA, pH 8.0 | Novocastra |
| CD43 | 1:10 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD45 (LCA) | 1:10 | 20 min EDTA, pH 8.0 | Dako |
| CD45RO | 1:100 | 20 min MW, citrate pH 6.0 | Dako |
| CD56 | 1:100 | 20 min MW, citrate pH 6.0 | Novocastra |
| CD57 | 1:40 | 20 min MW, citrate pH 6.0 | Becton Dickinson |
| CD68 | 1:700 | 10 min MW, citrate pH 6.0 | Dako |
| CD68 | 1:50 | 10 min MW, citrate pH 6.0 | Dako |
| CD79a | 1:20 | 20 min MW, citrate pH 6.0 | Dako |
| CD99 | 1:25 | 10 min MW, citrate pH 6.0 | Dako |
| CD138 | 1:100 | 20 min MW, citrate pH 6.0 | Serotec |
| Bcl-2 | 1:7 | 20 min MW, citrate pH 6.0 | Dako |
| Bcl-6 | 1:20 | 20 min EDTA, pH 8.0 | Dako |
| Cyclin–D1 | 1:50 | 20 min MW, citrate pH 6.0 | |
| Perforin | 1:20 | 20 min MW, citrate pH 6.0 | Novocastra |
| Granzyme-B | 1:20 | 20 min MW, citrate pH 6.0 | Novocastra |
| Alk | 1:10 | 20 min Dako retrievalsolution, pH 9.9 | Dako |
| HLA-DR | 1:100 | 20 min MW, citrate pH 6.0 | Dako |
| DBA44 | 1:100 | 20 min MW, citrate pH 6.0 | Dako |
| TRAP | 1:100 | 20 min MW, citrate pH 6.0 | Novocastra |
| vs38C | 1:100 | 20 min MW, citrate pH 6.0 | ICRF |
| Mum-1 | 1:200 | 20 min MW, citrate pH 6.0 | Santa Cruz |
| Pax-5 | 1:400 | 20 min MW, citrate pH 6.0 | Santa Cruz |
| Lysozyme | 1:3000 | No treatment/10 min trypsin | Dako |
| κ | 1:10,000 | No treatment | Dako |
| λ | 1:10,000 | No treatment | Dako |
| IgG | 1:3000 | 10 min Trypsin | Dako |
| IgM | 1:300 | 20 min Trypsin | Dako |
| IgD | 1:400 | 20 min MW, citrate pH 6.0 | Dako |
| IgA | 1:1000 | 10 min Trypsin | Dako |
| IgE | 1:50 | 10 min Trypsin | Dako |
| EBV-LMP-1 | 1:50 | 10 min MW, citrate pH 6.0 | Novocastra |
| HHV-8 | 1:50 | 20 min MW, citrate pH 6.0 | Novocastra |
| Parvovirus | 1:800 | 10 min MW, citrate pH 6.0 | Novocastra |
| Broad range keratin | 1:150 | 10 min Trypsin | Dako |
| EMA | 1:100 | 20 min MW, citrate pH 6.0 | Dako |
| HMFG1 | 1:800 | No treatment | ICRF |
| HMFG2 | 1:50 | No treatment | Novocastra |
| GCDFP* | 1:100 | 20 min MW, citrate pH 6.0 | Novocastra |
| ER | 1:200 | 20 min EDTA, pH 8.0 | Novocastra |
| PR | 1:100 | 20 min MW, citrate pH 6.0 | Biogenix |
| PSA | 1:2000 | No treatment | Dako |
| TTF-1 | 1:50 | 20 min MW, citrate pH 6.0 | Novocastra |
| PCNA | 1:50 | No treatment | Novocastra |
| Ki-67 | 1:50 | 20 min MW, citrate pH 6.0 | Novocastra |
| p53 | 1:50 | 10 min MW, citrate pH 6.0 | Novocastra |
| Mcm-2 | 1:50 | 20 min MW, citrate pH 6.0 | Gift from Dr Dilworth |
| Chromogranin* | 1:200 | No treatment | Biogenix |
| Synaptophysin* | 1:40 | 20 min MW, citrate pH 6.0 | Dako |
| Vimentin* | 1:1000 | No treatment | Sigma |
| S100* | 1:1000 | No treatment | Dako |
| Desmin* | 1:25 | 20 min MW, citrate pH 6.0 | Dako |
| Melan A* | 1:50 | 20 min MW, citrate pH 6.0 | Novocastra |
| HMB45* | 1:50 | No treatment | Dako |
| CK7* | 1:25 | 10 min MW, citrate pH 6.0 | Dako |
| CK20* | 1:100 | 10 min Trypsin | Dako |
| Thyroglobulin* | 1:1000 | No treatment | Dako |
| Calcitonin* | 1:800 | No treatment | Novocastra |
| Myoglobin* | 1:500 | No treatment | Dako |