Expected immunohistochemical and histochemical staining reactions in epithelioid malignant mesothelioma and adenocarcinoma
| Marker/stain | Mesothelioma | Adenocarcinoma |
|---|---|---|
| +, positive; −, negative. | ||
| *Positive in a small proportion of non-pulmonary adenocarcinomas. | ||
| †Nuclear staining in pulmonary adenocarcinoma and thyroid carcinoma. | ||
| ‡Positive in some renal, ovarian and endometrioid carcinomas. | ||
| §Positive in most renal and urothelial carcinomas. | ||
| CEA, Monoclonal carcinoembryonic antigen; TTF-1 thyroid transcription factor-1; WT1, Wilms tumour gene 1; PAS-D, periodic acid-Schiff-D. | ||
| Pan-cytokeratin | + | + |
| Cytokeratin 5/6 | + | −* |
| Calretinin | + | − |
| CEA | − | + |
| B72.3 | − | + |
| TTF-1 | − | +† |
| Ber-EP4 | − | + |
| D2-40 | + | − |
| WT1 | + | −‡ |
| Thrombomodulin | + | −§ |
| MOC-31 | − | + |
| BG-8 | − | + |
| CD15 (Leu-M1) | − | + |
| Periodic acid-Schiff without diastase | + or − | + |
| Periodic acid-Schiff with diastase (PAS-D) | − | + |
| Alcian blue | + | +/− |
| Alcian blue with hyaluronidase | − | If + without digestion, remains + after digestion |