Table 1 Major clinical studies of the prognostic value of disseminated tumour cells (DTC) detection in bone marrow (BM) by immunocytochemistry and prognostic value on disease and overall survival (univariate and multivariate analysis)
ReferenceSamplingMarkerNo. patientsDetection rate (%)Follow-up (mth)Disease free survivalOverall survival
UnivMultivUnivMultiv
Redding 198379SmearMUC11028NANANANA
Manegold 198880BiopsyCK/PKK1508NANANANA
Smear
Landys 199881BiopsyCK/AE1–AE3, KL1, CAM 5-212819240NANAYesNA
Salvadori 199082BiopsyCK/MBr112116.548NoNoNANA
Mathieu 199083BiopsyMUC/EMA, HMFG2931NoNoNoNo
CK/KL1, AE1–AE3, CAM5-2
Kirk 199084SmearMUC/anti-milk fat globulin LICR.LON.M8.4254834NoNANANA
Singletary 199185SmearCK/AE1, AE3, MAK-6713811NoNoNoNo
MUC/113F1, 260F9, 317G5
Cote 199186SmearMUC/C26, T164936.730YesYesNANa
CK/AE-1
Schlimok 199287CytospinCK18/CK21871839YesYesNANA
Harbeck 199488SmearCK1003834YesYesNoYes
MUC/EMA
Ménard 199489CytospinCK/MBr1, MBr8, CK18/CK2, MUC119731NANANANANA
Molino 199790CytospinCK/MBr1, MBr8, MOV8, MOV16 MluC11093136NoNoNoNo
Funke 199691CytospinCK18/CK223438NANANANANA
Diel 199692 93SmearMUC/TAG12 (2E11)72743.378YesYesYesYes
Mansi 199912 94SmearEMA35025.4150YesNoYesNo
Lyda 200095BiopsyCK/AE1–AE3, 35βH11 CAM 5-2543138YesNANANA
Untch 199996CytospinCK18/CK258128NoNoNoNo
Braun 200011CytospinCK/CK8,18,19 (A45 B/B3)5523636YesYesYesYes
Gerber 200120CytospinCK/CK8,18,19 (5D3)5543754YesYesYesYes
Gebauer200121SmearCK, MUC/EMA3964275YesYesYesYes
Kasimir-Bauer 200197CytospinCK/CK8,18,19 (A45 B/B3)1283424NANANANA
Naume 20045CytospinCK/AE1/AE38191349YesYesYesYes
Braun 20051VariousVarious470330.663YesYesYesYes
Bidard 20078CytospinCK/CK8,18,19 (A45 B/B3)6211550YesYesYesYes
  • CK, cytokeratin; Muc, mucin; EMA, epithelial membrane antigen; NA, not available.