Table 1 Sequence of sectioning and staining for sentinel lymph node for melanoma
SectionTreatment
Section 1 (first full section)H&E
Section 2S100
Section 3Spare
50 μm gap (+50 μm)
Section 4H&E
Section 5S100
Section 6Spare
Section 7Spare
Section 8Spare
Section 9Spare
50 μm gap (+100 μm)
Section 10H&E
Section 11S100
Section 12Spare
50 μm gap (+150 μm)
Section 13H&E
Section 14S100
Section 15Spare
50 μm gap (+200 μm)
Section 16H&E
Section 17S100
Section 18Spare
50 μm gap (+250 μm)
Section 19H&E
Section 20S100

  • In large or round lymph nodes, the 50 μm gap may be increased up to a maximum of 100 μm.