Assays for Clostridium difficile
| Target | Reference method | Other methods |
| C difficile toxin(s) | Cell-cytotoxicity assay | Toxin enzyme immunoassays; toxin membrane assays |
| C difficile | Culture | Common antigen (glutamate dehydrogenase) immunoassays |
| C difficile with the capacity to produce toxin(s) | Cytotoxigenic culture | Toxin gene PCR amplification techniques |