Protocols, reagents, and instrumentation used in the analysis
| Anti-ERα | Pretreatment protocol | Pretreatment instrument | Immunohistochemical staining reagents and protocol | Automated staining instrument |
|---|---|---|---|---|
| Dako monoclonal rabbit anti-ERα, clone EP1, FLEX ready-to-use | 20 min @ 97°C in Dako EnVision FLEX target retrieval solution, high pH | Dako PT link pretreatment module | Dako EnVision FLEX 20 min primary antibody 20 min EnVision FLEX/HRP 10 min DAB+ | Dako autostainer Link 48 |
| ERα component of the Dako ER/PR pharmDx kit (monoclonal mouse anti-ERα, clones 1D5 and ER-2-123) | 5 min @ 125°C in Dako EnVision FLEX target retrieval solution, low pH | Dako Pascal pressurised heating chamber | Dako EnVision+ /HRP, mouse 30 min primary antibody 30 min EnVision+/HRP 10 min DAB+ | Dako autostainer Link 48 |
| Ventana monoclonal rabbit anti-ERα, clone SP1, ready-to-use (CONFIRM) | 32 min mild CC1 | Ventana BenchMark XT | XT ultraView DAB kit | Ventana BenchMark XT |
ER, oestrogen receptor.