Table 2

Clinicopathological characteristics and genotype of the RHOA mutant gastric cancers

Case number	Gender	Age at diagnosis	Tumour localisation	Laurén phenotype	T-category	N-category	M-category	UICC stage	Tumour grade	KRAS codon 12 and 13	PIK3CA exon 9	PIK3CA exon 20	MSI status	EBV status	Helicobacter pylori status	RHOA mutation	RHOA exon	RHOA substitution mutation	Functional region—interaction with
1	F	44	Distal	Diffuse	T1b	N0	M0	IA	G2	WT	WT	WT	MSS	−	mv	c.125A>G, p.Y42C	2	Transition	Effector domain
2	F	38	Distal	Diffuse	T3	N1	M0	IIIA	G3	WT	WT	WT	MSS	−	−	c.169T>G, p.L57V	3	Transversion	PKN/PRK1
3	F	66	Distal	Diffuse	T1a	N0	M0	IA	G3	WT	WT	WT	MSS	−	−	c.169T>G, p.L57V	3	Transversion	PKN/PRK1
4	F	80	Distal	Diffuse	T4a	N2	M0	IIIB	G3	WT	WT	WT	MSS	−	−	c.65_66delinsGT, p.L22R	2	Deletion/Insertion	–
5	F	68	Distal	Diffuse	T4a	N3a	M0	IIIC	G3	WT	WT	WT	MSS	−	mv	c.206T>G, p.L69R#	3	Transversion	–
6	F	69	Proximal	Diffuse	T2	N0	M0	IB	G3	WT	WT	WT	MSS	−	−	c.125A>G, p.Y42C	2	Transition	Effector domain
7	M	90	Proximal	Diffuse	T3	N3	M0	IIIB	G3	WT	WT	WT	MSS	−	−	c.50G>A, p.G17E	2	Transition	GTP
8	M	68	Proximal	Diffuse	T3	N2	M0	IIIA	G3	WT	WT	WT	MSS	−	−	c.14G>A, p.R5Q	2	Transition	–
9	M	62	Distal	Diffuse	T3	N2	M0	IIIA	G3	WT	WT	WT	MSS	−	mv	c.109A>G, p.T37A#	2	Transition	Effector domain
10	F	72	Distal	Intestinal	T3	N3a	M0	IIIB	G3	WT	WT	WT	MSS	−	−	c.125A>G, p.Y42C	2	Transition	Effector domain
11	M	84	Distal	Intestinal	T2	N0	M0	IB	G3	WT	WT	WT	MSS	−	−	c.125A>G, p.Y42C	2	Transition	Effector domain
12	M	85	Distal	Intestinal	T2	N3a	M0	IIIA	G3	WT	WT	WT	MSS	−	−	c.70G>T, p.V24F	2	Transversion	–
13	M	66	Distal	Intestinal	T3	N0	M0	IIA	G3	WT	WT	WT	MSI	−	+	c.50delinsAA, p.G17Efs*24#	2	Deletion/Insertion	GTP
14	F	80	Distal	Unclassified	T3	N1	M0	IIB	G3	WT	WT	WT	MSS	−	−	c.169T>G, p.L57V	3	Transversion	PKN/PRK1
15	F	58	Distal	Unclassified	T1b	N3b	M0	IIB	G3	WT	WT	WT	MSS	−	−	c.125A>G, p.Y42C	2	Transition	Effector domain
16	M	59	Distal	Mixed	T4b	N3a	M0	IIIC	G3	WT	MUT	WT	MSS	+	+	c.14G>A, p.R5Q	2	Transition	–

#, hitherto unreported mutations; −, negative; +, positive; EBV, Epstein–Barr virus; GTP, guanosine triphosphate; MSI, microsatellite instable; MSS, microsatellite stable; mv, missing value; PKN/PRK1, protein kinase N1; UICC, Union for International Cancer Control; WT, wildtype.